MeltPro® MTB/STR Test Kit

MeltPro® Mycobacterium tuberculosis Streptomycin-resistance Mutation Test Kit

Mycobacterium tuberculosis Streptomycin-resistance Mutation Test is suitable for rapid, qualitative de-
tection of streptomycin-resistance mutations in Mycobacterium tuberculosis.

Mycobacterium tuberculosis Streptomycin-resistance Mutation Test is based on the Probe-based Melting
Curve Analysis. According to the change in T m value between targets and the corresponding wild-type
probe sequences, the test detects mutations in rpsL codon 43 and codon 88, rrs positions 513~517 and
positions 905~908, which reports streptomycin-resistance information. For each specimen, two tubes are
needed for the test, corresponding to the STR PCR Mix A and Mix B.

Detection region: mutations in rpsL codon 43 and codon 88, rrs positions513~517 and positions 905~908
(mutation rate 70%~90%)

Principle of Probe-Based Melting Curve Analysis

Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest T m value. With matched mutant-type target, it gives the lower T m value.
Mutation is detected as T m deviation (△T m ) compared to the wild-type hybrid.