MeltPro® MTB/INH Test Kit

MeltPro® Mycobacterium tuberculosis Isoniazid-resistance Mutation Test Kit

Mycobacterium tuberculosis Isoniazid-resistance Mutation Test is suitable for rapid, qualitative
detection of isoniazid-resistance mutations in Mycobacterium tuberculosis.

Mycobacterium tuberculosis Isoniazid-resistance Mutation Test is based on the Probe-based Melting
Curve Analysis. According to the change in T m value between targets and the corresponding wild-type
probe sequences, the test detects the mutations in ahpC promoter region (positions -44~-30 and -15~3),
inhA codon 94, inhA promoter region (positions -17~-8), and katG codon 315, which reports
isoniazid-resistance information.

Detection region: mutations in ahpC promoter region (positions -44~-30 and -15~3), inhA codon 94,
inhA promoter region(positions -17~-8) and katG codon 315 (coverage rate: 50%-90% of all isoniazid-resistant mutants)

Principle of Probe-Based Melting Curve Analysis

Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest T m value. With matched mutant-type target, it gives the lower T m value.
Mutation is detected as T m deviation (△T m ) compared to the wild-type hybrid.