PaxView® HPV16/18/Others MPCR-ULFA Kit

PaxView® HPV16/18/Others MPCR-ULFA Kit

 

Overview

Currently there are over 100 known Human Papillomavirus (HPV) genotypes associated with a number of diseases including many types of cancers, particularly cervical cancer. HPV types are often referred to as high-risk and low-risk, based on whether they put a person at risk for cancer. Two HPV types (16 and 18) cause 70% of cervical cancers and precancerous cervical lesions. Therefore, a sensitive and specific detection of HPV in cervical samples is a useful tool for the early diagnosis of epithelial neoplasia and anogenital lesions.

PaxView® HPV16/18/Others MPCR-ULFA Kit is designed for the simultaneous detection of two high-risk types (16, 18) and common positive of HPV using multiplex polymerase chain reaction (MPCR) and the ensuing, proprietary universal lateral flow assay (ULFA) which is based on DNA-DNA hybridization on nitrocellulose membranes.

 

Targets

HPV16, 18, Common positive

 

Specimens

Vaginal/Cervical Swab, Urine

 

Features and Benefits

Visually check the test result on the ULFA device

Simultaneously detect HPV high-risk(16 and 18) and common positive in one assay

Highly sensitive and fast detection (within 10 min)

 

Performances

DNA Extraction: 15 min

DNA Amplification: 2 hrs

ULFA Detection: 10 min

 

Product Storage

PaxView® DNA Extraction Kit: storage at 2~8℃

PaxView® HPV16/18/Others MPCR Kit: storage at -25~-15℃

PaxView® ULFA Kit: storage at 15~30℃  

 

References

1. J. Aveyard, M. Mehrabi, A. Cossins, H. Braven and R. Wilson. One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow device. 2007. Chem Commun. 41. 4251-4253.

2. A. Chua, C. Y. Yean, M. Ravichandran B. Lim and P. Lalitha. A rapid DNA biosensor for the molecular diagnosis of infectious disease. 2011. Biosensors and Bioelectronics. 26. 3825-3831.

3. G. Y. Lee, S. M. Kim, S. Y. Rim, H. S. Choi, C.S. Park, and J. H. Nam. Human papillomavirus (HPV) genotyping by HPV DNA chip in cervical cancer and precancerous lesions. 2005. Int J Gynecol Cancer. 15(1). 81– 7.